Significant difference on Bar/Line chart
The 'Significant difference' option is available under Advanced Settings. This option is available for Bar chart, Line chart and Bar-Line Combo chart.
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It is set to 'Off' by default.
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The legend referring to 'Significant Difference' can be found under the chart.
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The sample total is the base set by default. This can be changed under the Advanced Settings 'Base' option.

These icons display the 'Significant Difference'.
There are possibilities to change the significant difference base and to edit calculation, which are options available under Advanced Settings.